Current position
Fellow
Research interests
General
Molecular genetics of disease
resistance in plants. The molecular biology of tomato and its
interactions with the leaf mould fungus Cladosporium fulvum
and the vascular wilt fungus Fusarium oxysporum f.
sp. lycopersici . The roles of leucine-rich repeat proteins
in plants. The use of green fluorescent protein (GFP) as a protein
localisation tool in plants.
Specific
The
main focus of my research is to examine a number of key questions
about the interaction between the tomato Cf-9 and Cf-9B resistance
proteins and the corresponding C. fulvum Avr9 and Avr9B
avirulence proteins. Which parts of the Cf-9 and Cf-9B proteins
carry the specificity for recognition of Avr9 and Avr9B, respectively?
Which parts of the Cf-9 and Cf-9B proteins generate a signal in
response to Avr9 or Avr9B recognition? Is there a signalling partner
involved and what is its nature? The Cf-9 protein carries a number
of potential signals for endocytosis and protein degradation similar
to those in animals and yeast. What is the role of these signals
in plants and in resistance protein function in particular?
Other areas of specific research activity include the isolation and characterization of the tomato I-3 gene for resistance to Fusarium oxysporum f. sp. lycopersici, the perception f Phytophthora elicitins by plant cells, the sub-cellular localisation of resistance and partner proteins in plant cells and the mechanism of entry of pathogen effector proteins into plant cells.
Selected Publications
Barker CL, Baillie BK, Hammond-Kocack KE, Jones JDG, Jones DA (2006) Dominant-negative interference with defence signalling by truncation mutations of the tomato Cf-9 disease resistance gene. The Plant Journal 46, 385-389.
Barker CL, Talbot SJ, Jones JDG, Jones DA (2006) A tomato mutant that shows stunting, wilting, progressive necrosis and constitutive expression of defence genes contains a recombinant Hcr9 gene encoding an auto-active protein. The Plant Journal 46, 369-384.
Lim GTT, Wang G-P, Hemming MN, Basuki S, McGrath DJ, Carroll BJ, Jones DA (2006) Mapping the I-3 gene for resistance to fusarium wilt in tomato: Application of an I-3 marker in tomato improvement and progress towards the cloning of I-3. Australasian Plant Pathology 35, 671-680.
Takemoto D, Hardham AR, Jones DA (2005) Differences in cell death induction by Phytophthora elicitins are determined by signal components downstream of MAP kinase kinase in different species of Nicotiana and cultivars of Brassica rapa and Raphanus sativus. Plant Physiology 138, 1491-1504.
Takemoto D, Jones DA (2005) Membrane release and destabilization of Arabidopsis RIN4 following cleavage by Pseudomonas syringae AvrRpt2. Molecular Plant-Microbe Interactions 18, 1258-1268.
Wilson RJ, Baillie BK, Jones DA (2005) ER retrieval of Avr9 comprises its elicitor activity consistent with perception of Avr9 at the plasma membrane. Molecular Plant Pathology 6, 193-197.
Jones DA, Takemoto D (2004) Plant innate immunity – direct and indirect recognition of general and specific pathogen-associated molecules. Current Opinion in Immunology 16, 48-62.
Hemming MN, Basuki S, McGrath DJ, Carroll BJ, Jones DA (2004) Fine mapping of the tomato I-3 gene for fusarium wilt resistance and elimination of a co-segregating resistance gene analogue as a candidate for I-3. Theoretical and Applied Genetics 109, 409-418.
Takemoto D, Jones DA, Hardham AR (2003) GFP-tagging of cell components reveals the dynamics of subcellular re-organization in response to infection of Arabidopsis by oomycete pathogens. The Plant Journal 33, 775-792.
Panter S, Hammond-Kosack KE, Harrison K, Jones JDG, Jones DA (2002) Developmental control of promoter activity is not responsible for the onset of Cf-9B-mediated resistance to leaf mold in mature tomato plants. Molecular Plant-Microbe Interactions 15, 1099-1107.
Benghezal M, Wasteneys GO, Jones DA (2000) The C-terminal dilysine motif confers ER localization to type I membrane proteins in plants. Plant Cell 12, 1179-1201.
Jones
DA, Thomas CM, Hammond-Kosack KE, Balint-Kurti PJ, Jones JDG
(1994) Isolation of the tomato Cf-9 gene for resistance to Cladosporium
fulvum by transposon tagging. Science 266, 789-793.
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