The Molecular Biology Facility at the Research
School of Biological Sciences (RSBS MBF) is an important new element of
research infrastructure for the biological and biomedical sciences. It
replaces the Proteomics and Microarray Facility and, in conjunction with the Mass Spectrometry Facility, forms the Omics Facility.
It will provide long-term
support for researchers at the ANU and in the wider research community
of the ACT region.
The facility aims to enhance and support world-class research in molecular
biology within the Australian National
University (ANU). We do this by providing training in a range of techniques
and access to molecular biology resources that are not always affordable
to the individual group.
We have a variety of equipment (see below).
We also run small
training courses in a range of areas. Already we have trained
ANU researchers in real time PCR, RNA extraction and 2D
electrophoresis. We also run courses as part of the ANU
Winter School, managed by the College of Science.
The MBF is part of the ANU's vision of creating a centre of biotechnology
excellence here in Canberra. The University provided funding to establish
the MBF which complements the existing Biomolecular
Resource Facility (BRF) at the John
Curtin School of Medical Research.
Equipment available
The Facility is equipped
with a range of instruments to assist researchers in all
areas of molecular biology.
Details
of the equipment and other shared equipment on the ANU campus
can be found at the DOSSER
website.
Important:
Before using any equipment in the facility you must speak with Julie Christie to arrange access and training. Equipment is booked online via this
website.
Use
of certain machines will also require the provision of a
charge code. Students will need to get approval from their
supervisor before beginning work in the facility.
Click on the title for details and a list of relevant protocols and links for that item.
|
|
| PALM |
$10/hr |
| Rotorgene |
$10/session (usually 3hr) |
| Affymetrix scanner |
$5/hr |
| 2D electrophoresis |
$20-115/sample (Click here for
details) |
| Protein fractionation |
$143/sample for full pH range |
| BigDye |
$110/100 ml ($55/50 ml) |
| PALM consumables |
Click here to see consumables PDF |
|
|
| PALM |
$50/hr |
| Rotorgene |
$50/session (usually 3hr) |
| Affymetrix scanner |
$25/hr |
| 2D electrophoresis |
$50-288/sample (Click here for
details) |
| Protein fractionation |
$358/sample for full pH range |
| BigDye |
$110/100 ml ($55/50ml) |
| PALM consumables |
Click
here to see consumables PDF |
Operational Philosophy
• One time sample set: This
is for you if you need help with an experimental design or if you want
to analyse your own data but would like us to do ONE sample analysis first.
Sample prepared by client, run by Unit staff and simple interpretation
supplied as required.
• Training: After
appropriate training, people may operate instruments to acquire data from
their own samples.
• Collaboration: This
is for you if you want the Unit to be actively involved in the development
of methodology, preparation of samples, and interpretation of results,
leading up to a joint publication with the Unit staff member as an author.
• Supervision: You
can invite a Unit staff member to join your supervisory panel. You will
then work with this person for the duration of your research programme.
There is an expectation that the supervisor will be an author on relevant
publications.
• Acknowledgments: Whenever
data derived from the use of the instruments in the RSBS Molecular Biology
Facility is published, it should be formally acknowledged, unless there
is co-authorship. We also ask that a copy of all such published work be
provided to the Unit for incorporation into the Annual Report.
(Download
Operational Philosophy as pdf)
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