The GPRIME
Package
Current version 1.00
posted 9th July 1997.
This package contains
two series of programs to help with the design of PCR primers
for an aligned group of related nucleotide sequences. These sequences
must be provided in a single file in PIR format, and this is obtained
most conveniently using CLUSTAL V or W. This version will handle
up to 20 sequences, each of 10000 nucleotides, and comprises:
- programs to locate primer sites shared by all sequences
in the group;
- programs to locate primer sites that will separate two sub-sets
of the sequences;
- programs that check for sites that have problems due to
self complementarity and/or self annealing
This software is at
the Beta test stage and we wish to hear of problems, criticisms
of the instructions, etc. Suggestions for improvements and extensions
are welcome.
The programs were written
in the Lahey ELF90 version of FORTRAN 90, and will operate under
DOS 3.3 et seq. They do not require WINDOWS or a numerical co-processor.
The archive contains 2
files README.TXT and GPRIME.EXE. The first is an ASCII file. The
other is a self extracting BINARY file and IT MUST BE TRANSFERRED
IN BINARY MODE. The file size is approx 0.6 Mb.
DOWNLOAD
programs here
The authors are:
John Armstrong, John.Armstrong@anu.edu.au
Adrian Gibbs, Adrian.Gibbs@anu.edu.au
Georg Weiller, Georg.Weiller@anu.edu.au
Research School of Biological
Sciences, Institute of Advanced
Studies, A.N.U., P.O.
Box 475, A.C.T., 2601.
ph. 61 (0)6 249 5916;
fax. 61 (0)6 249 4437;
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